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Wiley Online Library requires cookies for authentication and use of other site features; therefore, cookies must be enabled to browse the site.Detailed information on how Wiley uses cookies can be found in our Privacy Policy.The polyglutamine expansion in huntingtin (Htt) protein is a cause of Huntington's disease (HD).Htt is an essential gene as deletion of the mouse Htt gene homolog (Hdh) is embryonic lethal in mice.Except for the extreme amino-terminus, with its adjacent poly Q region and proline-rich segments, the entire ~350-k D protein is predicted to be composed of 36 α-helical HEAT repeats.Increasing evidence indicates that Htt functions as a molecular scaffold that is able to organize a variety of signaling complexes [1, 2].These software can provide close enough approximation to estimate a wide range of protein conformations that can be studied for a specific biological function.Protein folding is one of the most important parameters to study in structural biology, therefore there is a need for improved computational methods in protein folding predictions.

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Consistent with the genotype of the generated cells, we detected a protein of predicted size (~350 k D) in lysates from the Hdh-HET cells, but not in lysates from Hdh-KO lines (Fig 2).

Transfected cells were then cultured for four to six weeks until immortalized Hdh floxed/ and Hdh floxed/- mouse embryonic fibroblasts (MEFs) were obtained (Fig 1).

To recombine the Hdh floxed allele, immortalized Hdh floxed/ and Hdh floxed/- MEFs were infected with Lenti-NLS-GFP-Cre virus encoding nuclear-targeted GFP-Cre fusion protein [19] (Fig 1).

Htt is expressed ubiquitously in humans and rodents, with the highest levels found in CNS neurons and the testes [3–5]. All vertebrate isoforms of Htt, but not Drosophila Htt, contain an amino-terminal poly Q region.

Intracellularly, Htt is associated with various organelles, including the nucleus, endoplasmic reticulum (ER) and Golgi complex [6–8]. Complete knockout of the mouse Htt gene (Hdh) causes embryonic death before day 8.5 (E8.5, before gastrulation and the formation of the nervous system) [10–12].

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