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Consistent with the genotype of the generated cells, we detected a protein of predicted size (~350 k D) in lysates from the Hdh-HET cells, but not in lysates from Hdh-KO lines (Fig 2).
Transfected cells were then cultured for four to six weeks until immortalized Hdh floxed/ and Hdh floxed/- mouse embryonic fibroblasts (MEFs) were obtained (Fig 1).
To recombine the Hdh floxed allele, immortalized Hdh floxed/ and Hdh floxed/- MEFs were infected with Lenti-NLS-GFP-Cre virus encoding nuclear-targeted GFP-Cre fusion protein  (Fig 1).
Htt is expressed ubiquitously in humans and rodents, with the highest levels found in CNS neurons and the testes [3–5]. All vertebrate isoforms of Htt, but not Drosophila Htt, contain an amino-terminal poly Q region.
Intracellularly, Htt is associated with various organelles, including the nucleus, endoplasmic reticulum (ER) and Golgi complex [6–8]. Complete knockout of the mouse Htt gene (Hdh) causes embryonic death before day 8.5 (E8.5, before gastrulation and the formation of the nervous system) [10–12].